Interaction of Titanium Dioxide Nanoparticle with Human Serum Albumin: a Spectroscopic Approach

نویسندگان

  • MOHD JAHIR KHAN
  • MOHAMAD YUSOF MASKAT
چکیده

Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by UV-vis and fluorescence spectroscopies. Results: The characterization result demonstrated that TiO2NPs were somewhat spherical with average diameter of ~32 nm. UV-vis and fluorescence spectroscopic studies showed that NPs form ground state complex with HSA. Conclusion: UV-vis and fluorescence spectroscopy depicted the formation of HSA-TiO2NPs complex induced conformational changes in human serum albumin.

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تاریخ انتشار 2014